Gherardi g, angeletti s, panitti m, pompilio a, di bonaventura g, crea f, et al. This chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Prep station entering target slide and vitek 2 ast test cards duration. Jan 25, 2016 in diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. Place a small barcode label near the top of a 3ml vitek saline tube. I started my career with algorithms for speeding up some of the most frequent operations performed by object oriented programs, namely, method dispatch and type tests. The information is then gathered and formatted into many vital reports, including epidemiology and statistical reports. Vitek 2 technology represents a smarter way to automate idast testing. Nov 30, 20 the reliability of using vitek 2 compact system to detect 1. Vitek 2 identification id cards let you easily and safely test and identify a wide range of clinically relevant organisms. The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana henry kwadwo hackman1, george oseiadjei1. Modifications by biomerieux can be made without prior notice biomerieux, the blue logo, advanced expert system, bioart, densichek, myla, vilink and vitek are used, pending andor registered trademarks belonging to biomerieux, or one of its subsid iaries, or one of its. Comparative evaluation of the vitek2 compact and phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of gramnegative and grampositive isolates. Place assembled card and transfer tube on filling stand with the long portion of the transfer tube into the test tube.
Used biomerieux vitek 2 compact microbiology for sale. Package insert contains system performance data and describes testing principle and procedure, qc recommendations, and limitations. For some isolates, other conventional methods such as api biome. Use of the vitek 2 system for rapid identification of clinical. The innovative vitek 2 microbial identification system includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal training time. Vitek 2 xl vitek 2 compact vitek 2 0118 93192002gbe document andor pictures not legally binding.
Vitek virtuoso series operation manual pdf download. Evaluation of the vitek 2 system for identification and. Generating an epub file may take a long time, please be patient. We evaluated the ability of the vitek 2 system biomerieux, inc. Description of the biomerieux vitek 2 gram negative susceptibility test cards, astgn04, 20pk. With this technique, more data is analyzed, which increases results and accuracy. Choose isolate prepare organism suspension and ensure correct mcfarland standard with densichek plus vitek 2 compact. Mandell, douglas and bennetts principle and practice of infectious diseases. The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the vitek 2 instrument and the smart carrier station. The course the routine use of vitek 2 compact has been designed for those who need to operate the instrument in a routine mode without having to perform expert tasks. Us environmental protection agency office of pesticide programs.
Vitek 2 instrument user manual i 51073110en1 general warnings important. This option is added to the microbiology lrmi menu by the kids installation process. Shop online for a wide selection of microbiologics vitek 2 gp comprehensive qc set microorganism sets for culture purposes and qc applications. The system reads the latest generation vitek test cards containing 64 wells to ensure accuracy every 15 minutes using three different wavelengths. The efficiency of the vitek 2 compact system relies on the advanced colorimetry technology. To inoculate the vitek 2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. Vitek 2 yeast caspofungin is a quantitative test intended for use with the vitek 2 and vitek 2 compact systems as a laboratory aid in the determination of in vitro susceptibility to antifungal agents. The fundamentals of vitek 2 compact operations are presented through lessons, tutorials and exercises to enable you to take the most out of the instrument. The quiz the routine use of vitek 2 compact knowledge assessment quiz has been designed for those who need to use the vitek 2 compact in a daily routine. Pdf vitek 2 automated identification system and kocuria.
The quiz enables you to measure your level of proficiency on the routine use of the software. Microbial identification using vitek 2 pdf pdadhi parenteral. Features of the biomerieux vitek 2 gram negative susceptibility test cards. The vitek 2 advanced expert system software is like having an expert advisor standing by your side. Realtime pcr detection of shigatoxin producing escherichia coli stec, serotype o157 and non o157 in fresh produce and food with nono157 isolation and identification revision.
Fast, accurate, resistanceoriented susceptibility test results. Used biomerieux vitek2 compact microbiology analyzer for. Biomerieux vitek 2 gram negative susceptibility test cards. Ensure highperformance products, reproducible clinical test results and consistent microbiological evaluation of pharmaceuticals and food with thermo scientific cultiloops quality control organisms. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of capacity and automation. Use of the vitek 2 system for rapid identification of. The aim of this study was to compare the application of phenotypic and molecular tools in bacterial identification on the example of gramnegative. The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana henry kwadwo hackman1, george osei. The reliability of using vitek 2 compact system to detect 1. Customer training course book and instrument user manual are maintained. Performance of vitek2 compact and overnight microscan. Headtohead comparison of microflex lt and vitek ms systems. Vitek2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures.
I proposed a compact solution to the dispatching problem in. Thermo scientific vitek 2 qc sets include a readytouse set of qc organisms in cultiloops for. Id and ast approach from positive blood culture bottles using vitek 2 compact. We evaluated the ability of the vitek 2 system biomerieux, inc, hazelwood, mo. It is a fully automated system that eliminates the inherent bias of manual mic determination. Quality control summary vitek2 compact, manual dilution inoculum density control was monitored using the densichek2 instrument. Package insert is located in the supervisors office.
Vitek manual table of contents mount sinai hospital. A broad choice of methods is available, based on both phenotypic and molecular properties of microbes. Malditof ms versus vitek 2 anc card for identification of. Virtuoso series security camera pdf manual download. Vitek 2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures. Observa is an information management system that stores the test data generated by the vitek 2 or vitek 2 compact. Application proper use of the instrument is the responsibility of trained.
Use of the vitek 2 system to identify and test the antifungal. Vitek 2 automated identification system and kocuria kristinae. Use, maintenance and quality control procedures scope the purpose of this sop is to describe the procedures for the preparation and identification of test microorganisms test microbes and quality control organisms using the vitek 2 compact instrument. Matrixassisted laser desorption ionization timeofflight mass spectrometry malditof ms is an accurate, rapid and inexpensive technique that has initiated a revolution in the clinical microbiology laboratory for identification of pathogens. Several manual and commercial identification methods have been developed and are. The vitek 2 advanced expert system software is like having an.
A study was conducted to evaluate the new vitek 2 system biomerieux for identification and antibiotic susceptibility testing of grampositive cocci. Any other name or trademark is the property of its respective owners biomerieux. Hazelwood, mo, usa objective this chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Office of pesticide programs microbiology laboratory. Headtohead comparison of microflex lt and vitek ms. Place filling stand with the assembled test kit into filling module. The vitek 2 compact system is an automated microbiology bacterial identification. Evaluation of the vitek 2 system for identification and antimicrobial. Performance of vitek2 compact and overnight microscan panels. Laboratorybased evaluation of the colorimetric vitek2 compact. United states department of agriculture agricultural. Comparative evaluation of the vitek 2 compact and phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of gramnegative and grampositive isolates.
Application of routine diagnostic procedure, vitek 2 compact. Designed for vitek 2 automated instruments, vitek 2 id cards help ensure you can rapidly provide accurate, relevant results. April 1996 dhcp laboratory vitek literal interface la5. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of. Malditof ms versus vitek 2 anc card for identification of anaerobic bacteria background. Vitek 2 yeast caspofungin is designed for antifungal susceptibility testing of candida species. Principles the vitek 2 is an automated microbiology system utilizing growth based technology. The vitek 2 system nextgeneration platform provides greater automation while increasing safety and eliminating repetitive manual operations. Scan cassette worksheet at the work station vitek 2 is designed to make your idast workflow as rapid and reliable as possible, while providing maximum flexibility and full traceability.
Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. Us environmental protection agency office of pesticide. The configuration that you have purchased is adapted to the legislation. The vitek 2 compact system biomerieux,france was used for confirmation of identified bacteria 12. Vitek 2 uses unique id and ast cards the size and shape of a playing card.
Manufacturer alerts and bulletins are located the supervisors office. Vteh series 4, 8, and 16 channel digital video recorders. From the above results we can conclude that vitek 2 compact is an automated system and gives more accurate results. Clinical isolates of staphylococcus aureus n 100, coagulasenegative staphylococci. Vitek 2 susceptibility results obtained with the astp507 card and analysed with the advanced expert system software v. The reliability of using vitek 2 compact system to detect. When compared to sequencing and biochemical testing, the vitek ms correctly identified the scientific group or family 93. Advances in life science and technology issn 22247181 paper issn 2225062x online vol. Performance of the vitek 2 system software version 5.
This biomerieux vitek 2 compact with dc7100usdt hp compaq computer is. Ergonomic analysis comparison of the vitek 2 and vitek 2 compact. Principles of the vitek 2 is an automated microbiology system utilizing growthbased technology. It is concluded that the new vitek 2 gp identification card provides reliable results for the. Rapid reliable identification of these organisms is essential for accurate diagnosis and prompt effective treatment. Staphylococci are an increasing cause of bloodstream infections. To inoculate the vitek2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. Subculture frozen or freeze dried isolates twice before setting up any vitek 2 cards. Vitek 2 compact identification cards for example, gp, anc, bcl, and gn. Quality control summary vitek2, auto and manual dilution a s an additional qc study was performed with the vitek2 compact, the secondary option, at three sites, with the results in the following tables.